Virus:Article Title: Multilevel regulation of c-di-GMP biosynthesis by cAMP signaling increases Shigella sonnei fitness and pathogenicity in response to bile salts.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Strains used in this study This paper see Table S3 Chemicals, peptides, and recombinant proteins Tryptone Oxoid Cat# LP0042B Yeast extract Oxoid Cat# PL0021 NaCl Macklin Cat# S805275 Agar Macklin Cat# A800728 Porcine bile salts Sigma-Aldrich Cat# B8631 Sodium chenodeoxycholate Sigma-Aldrich Cat# C8261 Sodium cholate MedChemExpress Cat# HY-N0324A Sodium deoxycholate MedChemExpress Cat# HY-N0593A Lithocholylglycine sodium MedChemExpress Cat# HY-116374A X-gal Solarbio Cat# X8050 IPTG Solarbio Cat# I8070 Chloramphenicol TargetMol Cat# T1205 Kanamycin Macklin Cat# K885955 Ampicillin Macklin Cat# A830931 Streptomycin Sulfate Solarbio Cat# S8290 Protein Ladder Thermofisher Cat# 26616 EDTA Solarbio Cat# E8040 Crystal violet Sigma-Aldrich Cat# C6158 Sodium dodecyl sulfate Sigma-Aldrich Cat# L6026-50G Na 2 HPO 4 Sinopharm Cat# 20040618 β-mercaptoethanol Aladdin Cat# M274256 MgSO 4 Aladdin Cat# M475695 NaH 2 PO 4 Macklin Cat# M888173-500g KCl Macklin Cat# P708031-5g MnCl 2 Macklin Cat# 767273-5g Chloroform Hushi Cat# 10006818 ONPG Solarbio Cat# IO0050 PBS buffer Macklin Cat# 917808 Tris Beyotime Cat# ST760 50 × TAE buffer Solarbio Cat# T1060 5 × TBE buffer Solarbio Cat# T1050 Dulbecco’s Modified Eagle’s Medium-high glucose (DMEM) Sigma-Aldrich Cat# D6046 Fetal Bovine Serum Sigma Cat# F4135 DMSO Sigma-Aldrich Cat# D5879 5 ′ -GTP disodium salt MCE Cat# HY-W010737 ATP Selleck Cat# S5260 3 ′ ,5 ′ -cAMP Aladdin Cat# A122959 c-di-GMP InvivoGen Cat# tlrl-nacdg Acetyl phosphate Sigma-Aldrich Cat# A0262 Critical commercial assays 2 × Phanta Flash Master Mix Vazyme Cat# P520-01 (Continued on next page) 14 Cell Reports 44, 116306, October 28, 2025 ..
Recombinant:Article Title: Multilevel regulation of c-di-GMP biosynthesis by cAMP signaling increases Shigella sonnei fitness and pathogenicity in response to bile salts.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Strains used in this study This paper see Table S3 Chemicals, peptides, and recombinant proteins Tryptone Oxoid Cat# LP0042B Yeast extract Oxoid Cat# PL0021 NaCl Macklin Cat# S805275 Agar Macklin Cat# A800728 Porcine bile salts Sigma-Aldrich Cat# B8631 Sodium chenodeoxycholate Sigma-Aldrich Cat# C8261 Sodium cholate MedChemExpress Cat# HY-N0324A Sodium deoxycholate MedChemExpress Cat# HY-N0593A Lithocholylglycine sodium MedChemExpress Cat# HY-116374A X-gal Solarbio Cat# X8050 IPTG Solarbio Cat# I8070 Chloramphenicol TargetMol Cat# T1205 Kanamycin Macklin Cat# K885955 Ampicillin Macklin Cat# A830931 Streptomycin Sulfate Solarbio Cat# S8290 Protein Ladder Thermofisher Cat# 26616 EDTA Solarbio Cat# E8040 Crystal violet Sigma-Aldrich Cat# C6158 Sodium dodecyl sulfate Sigma-Aldrich Cat# L6026-50G Na 2 HPO 4 Sinopharm Cat# 20040618 β-mercaptoethanol Aladdin Cat# M274256 MgSO 4 Aladdin Cat# M475695 NaH 2 PO 4 Macklin Cat# M888173-500g KCl Macklin Cat# P708031-5g MnCl 2 Macklin Cat# 767273-5g Chloroform Hushi Cat# 10006818 ONPG Solarbio Cat# IO0050 PBS buffer Macklin Cat# 917808 Tris Beyotime Cat# ST760 50 × TAE buffer Solarbio Cat# T1060 5 × TBE buffer Solarbio Cat# T1050 Dulbecco’s Modified Eagle’s Medium-high glucose (DMEM) Sigma-Aldrich Cat# D6046 Fetal Bovine Serum Sigma Cat# F4135 DMSO Sigma-Aldrich Cat# D5879 5 ′ -GTP disodium salt MCE Cat# HY-W010737 ATP Selleck Cat# S5260 3 ′ ,5 ′ -cAMP Aladdin Cat# A122959 c-di-GMP InvivoGen Cat# tlrl-nacdg Acetyl phosphate Sigma-Aldrich Cat# A0262 Critical commercial assays 2 × Phanta Flash Master Mix Vazyme Cat# P520-01 (Continued on next page) 14 Cell Reports 44, 116306, October 28, 2025 ..
Modification:Article Title: Multilevel regulation of c-di-GMP biosynthesis by cAMP signaling increases Shigella sonnei fitness and pathogenicity in response to bile salts.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains Strains used in this study This paper see Table S3 Chemicals, peptides, and recombinant proteins Tryptone Oxoid Cat# LP0042B Yeast extract Oxoid Cat# PL0021 NaCl Macklin Cat# S805275 Agar Macklin Cat# A800728 Porcine bile salts Sigma-Aldrich Cat# B8631 Sodium chenodeoxycholate Sigma-Aldrich Cat# C8261 Sodium cholate MedChemExpress Cat# HY-N0324A Sodium deoxycholate MedChemExpress Cat# HY-N0593A Lithocholylglycine sodium MedChemExpress Cat# HY-116374A X-gal Solarbio Cat# X8050 IPTG Solarbio Cat# I8070 Chloramphenicol TargetMol Cat# T1205 Kanamycin Macklin Cat# K885955 Ampicillin Macklin Cat# A830931 Streptomycin Sulfate Solarbio Cat# S8290 Protein Ladder Thermofisher Cat# 26616 EDTA Solarbio Cat# E8040 Crystal violet Sigma-Aldrich Cat# C6158 Sodium dodecyl sulfate Sigma-Aldrich Cat# L6026-50G Na 2 HPO 4 Sinopharm Cat# 20040618 β-mercaptoethanol Aladdin Cat# M274256 MgSO 4 Aladdin Cat# M475695 NaH 2 PO 4 Macklin Cat# M888173-500g KCl Macklin Cat# P708031-5g MnCl 2 Macklin Cat# 767273-5g Chloroform Hushi Cat# 10006818 ONPG Solarbio Cat# IO0050 PBS buffer Macklin Cat# 917808 Tris Beyotime Cat# ST760 50 × TAE buffer Solarbio Cat# T1060 5 × TBE buffer Solarbio Cat# T1050 Dulbecco’s Modified Eagle’s Medium-high glucose (DMEM) Sigma-Aldrich Cat# D6046 Fetal Bovine Serum Sigma Cat# F4135 DMSO Sigma-Aldrich Cat# D5879 5 ′ -GTP disodium salt MCE Cat# HY-W010737 ATP Selleck Cat# S5260 3 ′ ,5 ′ -cAMP Aladdin Cat# A122959 c-di-GMP InvivoGen Cat# tlrl-nacdg Acetyl phosphate Sigma-Aldrich Cat# A0262 Critical commercial assays 2 × Phanta Flash Master Mix Vazyme Cat# P520-01 (Continued on next page) 14 Cell Reports 44, 116306, October 28, 2025 ..
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